Feasibility of UV-Vis Spectral Fingerprinting Combined with Chemometrics for Rapid Detection of Phyllanthus niruri Adulteration with Leucaena leucocephala

Phyllanthus niruri is widely used in Indonesia as immunostimulant. The morphology of Leucaena leucocephala leaves similar to that P. leaves. L. easy find and collect because it distributed the world. Therefore, likely could be adulterated with leucocephala. identification authentication important ensure raw materials are original without any substitution or mixture other plants causing inconsistencies their efficacy. In this paper, we described feasibility UV-Vis spectral fingerprinting chemometrics for rapid method detection spectra samples measured interval 200-800 nm signal smoothing followed by standard normal variate were pre-processing data. Principal component analysis (PCA)with absorbance data from pre-processed range 250-700 variables distinguish PCA discriminant (DA) successfully classified mixed 5, 25, 50% luecocephala into respective groups (96.81%). We also employed soft independent modelling class analogy (SIMCA) found 88.3% correctly groups. A combination spectroscopy chemometrics, such PCA-DA SIMCA, first time